2nd International Conference on Advances in Skin, Wound Care and Tissue Science November 9-10, 2017 Frankfurt, Germany

Biography:

Lim Fui Ping is a lecturer at the Alice Lee Centre for Nursing Studies, National University of Singapore. She obtained her Master degree in Nursing at the University of Sydney, Australia and is presently pursuing her doctorate degree at Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore. She has a long-standing interest in translational research, and in the development of novel cell-based therapies to improve wound healing, with a particular focus on chronic and non-healing wounds.

Abstract:

Aim: This study investigates the healing potential of Cord Lining Mesenchymal Stem Cells       (CLMSC) in full- thickness dermal wounds of db/db diabetes mice.

Methods: 20 mice were randomly assigned to two arms; the control group received placebo treatment (sham media) while experiment group received CLMSC via intra-peritoneal (IP) injection. Two full-thickness wounds, each sized 10mm X 10mm, were created on the back of the mice. Wound areas were analyzed weekly with Image J TM software. Tissues were harvested at point of termination for immune-profiling and histological analysis. Pro-inflammatory and anti-inflammatory cytokines were quantified to determine the immunomodulation properties of the CLMSC.

Results: The CLMSC-treated wounds, compared with the control group, showed a significant increase in the percentage of wound closure from day 14 onwards and eventually achieved 100% closure of the wound sooner that the control group by an average of 3.7 days. The mice treated with CLMSC have a shorter wound closure time (mean closure day: 19.8 days) as compared to the control group (mean closure day: 23.5 days). Our histology analysis of repair revealed that CLMSC treatment enhanced re-epithelization with a fully differentiated multi-layered epithelium developed from Day 21 onwards, while the epithelium regenerated in control group was not fully differentiated at the same time point. Our systemic analysis on immunomodulation indicated an upregulation of pro-inflammatory serum cytokines (GM-CSF and IFN-gamma) in CLMSC treatment group during the first 7 days of injury to allow infiltration of inflammation cells. There is upregulation of anti-inflammatory serum cytokines (IL-10) to halt the inflammatory activity after 7 days of injury

Conclusions: Our preliminary findings are indicative of the positive effect of CLMSCs on diabetic wound healing, with the potential of CLMSC as an anti-inflammatory agent.

Biography:

Ryan Moseley graduated from Swansea University with a BSc (Honours) Degree in Biochemistry. Later, he obtained his PhD from the School of Dentistry, University of Wales College of Medicine, examining the role of oxidative stress in periodontal disease. Dr Moseley continues his research at Cardiff University, where he is currently a Reader in Tissue Repair and Director of the CITER MSc Programme in Tissue Engineering. Dr. Moseley’s research focusses on the mechanisms underlying dermal and oral wound healing during health and disease; and the development of stem cell-, biomaterial- and pharmaceutical-based strategies to address impaired healing in these tissues.

Abstract:

Excessive dermal scarring/fibrosis possess major challenges to Healthcare Services worldwide.  This is confounded by existing therapies being unsatisfactory at preventing or arresting fibrosis.  Therefore, there is significant need for novel anti-fibrotic therapies with improved efficacy. The anti-cancer drug, ingenol mebutate (IngM), is licensed for actinic keratosis and non-melanoma skin cancers. IngM also stimulates exceptional healing and reduced scarring in treated skin, implying IngM possesses potent anti-fibrotic properties. As transforming growth factor-β1 (TGF-β1)-driven, dermal fibroblast-myofibroblast differentiation is pivotal to scarring outcomes, fibroblasts and myofibroblasts represent viable IngM targets. This work evaluated IngM efficacy against normal (dermal) and pathological (keloid) fibroblasts/myofibroblasts; and its mechanisms of action.

Human dermal and keloid fibroblasts were treated with IngM (0-10µg/mL) and rhTGF-β1 (10ng/mL); and assessed for myofibroblast differentiation by immunocytochemistry/QRT-PCR for -smooth muscle actin (α-SMA) expression/stress fibre formation; QRT-PCR/Western blotting of other myofibroblast-/extracellular matrix (ECM)-related genes/proteins; hyaluronan synthesis/assembly (ELISA, hyaluronan synthase QRT-PCR, pericellular localization); and Microarrays/protein validation analysis.

Although IngM does not inhibit dermal fibroblast-myofibroblast differentiation, IngM alters myofibroblast pro-fibrotic ECM deposition due to enhanced degradation of type I collagen by up-regulated matrix metalloproteinases (MMP-1, MMP-9). IngM also increases myofibroblast hyaluronan synthesis by hyaluronan synthase-2. Although reduced MMPs and hyaluronan are hallmarks of keloid scars, increased MMP/hyaluronan levels were also identified in IngM-treated keloid fibroblasts; in addition to reversed keloid fibroblast-myofibroblast formation and reduced type I collagen expression.

Therefore, IngM modulates dermal/keloid fibroblast differentiation and behaviour, inducing scar tissue resolution. Such findings support IngM development/repurposing as a novel anti-fibrotic therapeutic against normal and pathological dermal scarring.

Biography:

Gulsan Ara Sathi has her expertise in tissue regeneration and passion in improving the disease control and treatment facility (Sasaki & Sathi 2017). Her new idea provides new findings in developmental biology (Sathi GA, 2017). She also works as a part of different creative biomimetic materials evaluation study (Taketa 2015, Farahat 2017). She has built up her such view in regenerative field after years of experience in research, evaluation, teaching and both in hospital and education institutions.

Abstract:

A problem increasing day by day is that millions of people suffer from wounds including skin wounds, surgical or traumatic disruption. Although, suture is still the main device to close the wounds, it has some disadvantages such as granuloma formation, inflammatory reactions and scar formation, which is sometimes aesthetically difficult to accept. Therefore, tissue adhesives have been good alternatives as supporting materials. This fact is based on their attractive properties including reduction of surgery time, mitigation of infection, preventing leakages, easy application, no removal requirements, less pain and great cosmetic results.  Surgical adhesives can be made from natural polymers includes chitin, chitosan, gelatin, collagen, alginate. Among the natural polymers, sodium alginate is a natural linear polysaccharide and has biocompatibility, non-toxicity, non-immunogenicity, biodegradability, antimicrobial activity, and can be simply gelled with divalent cations. Also it has been used in a number of biomedical applications and drug delivery. In recent years essential nutrient metal element get a huge interest in the field of regenerative medicine. Chromium (Cr) is an essential trace element. Trivalent oxidation state of the chromium (Cr3+) is the most stable form in biological systems and beneficial effects of Cr3+ ion is widely studied that it regulates the IGF-1and collagen synthesis. However, weather the Cr3+ has function on wound healing is still unclear. In this study, chromium ion induced alginate hydrogel base bio adhesives were designed for surgical wound close. We prepared 0.3% (W/V) alginate hydrogel using the trivalent chromium solution and animal experiment was carried out.  Different size surgical wound along with bleeding was made on mouse ventral and dorsal surface and then closed the wound using either alginate-Cr solution or traditional suture materials. After 1 week and 2 weeks a protruding tissue at the wound closure area can be seen in control group, which was not looks nice aesthetically, whereas it was very difficult to find out the wound region in experimental sample confirmed its aesthetical property. This pilot study suggests that this new materials have a great potential as wound closing materials particularly where aesthetical appearance is more.

Biography:

Denis Barritault graduated in Physics, completed his PhD in biochemistry in Paris University. Post-doctoral in molecular immunology at Pasteur Institute and NYU as NIH Fogarty Fellow he joined INSERM unit in Paris as developmental biologist. He made the first description and patents of FGF extracted from retina in 1979 and 82 as skin and cornea healing agent, became full professor at Paris-Est University in 1985, founded and directed a CNRS Laboratory on cell and tissue regeneration until 2003. He is now President of OTR3, Emeritus professor, honorary director CRRET CNRS unit and author in over 200 publications and 30 patents. He succeeded in transforming his research in basic science into product to treat patients; one to treat skin wound the other for corneal ulcer. Several other products are now in development for new indication in regenerative medicine.

Abstract:

ReGeneraTing Agents (RGTAs) are polysaccharide engineered to mimic heparan sulfate. When introduced into a damage tissue they induce a regeneration process by restoring -the proper cellular microenvironment. CACIPLIQ20 is a formulation based on RGTA OTR4120 polymer adapted to skin lesions. We present selected cases illustrating the efficacy and patient benefit of this therapeutic class of products considered as a new branch of regenerating medicine. The examples will cover hand trauma cases, ischemic, burns, plastic and reconstructive surgery, and many chronic ulcers.

As for hand surgery, in the emergency setting, the spectrum of cases is very wide ranging from minor injuries to major amputations comprising fresh cases as well as secondary referrals. Dealing with open (non-healing) wounds, infections and amputations especially in smokers is one of the challenges yet to have a satisfactory and predictable outcome

We describe the cases of 14 patients with palmar and digital injuries, of which six were considered for amputation (n=6), five had full thickness skin graft loss or burns (n= 5) and three developed or had severe infections (n= 3). After surgery, CACIPLIQ20® was used to stop or avoid severe outcomes. In all 14 cases, CACIPLIQ20® significantly improved the outcomes of the hand injuries in terms of range of motion despite skin, tendon and bone healing by secondary intention. CACIPLIQ20® showed a significant improvement in the healing of hand injuries as well as in terms of functional recovery and therefore can be used at earlier or even later stages to prevent an adverse outcome. As for burns, cases of all grades will be presented. Altogether, these cases should shed light into a new vision and evaluation on the cost effectiveness and patient benefits of this new technology.

Biography:

Sandeep Shrivastava is working as Professor of Orthopaedics. He is the CEO Hospitals at J.N. Medical School, Datta Meghe Institute of Medical Sciences University Wardha, India. Since last 7 years, he is involved in the development of the clinical applications of PRP in Wound management. He has 62 Publications and has presented his work across the globe in more than 75 Conferences and forums. He holds six copyrights on his different inventions and innovations.

Abstract:

The complex wounds associated with infections, necrosis, exposed tendons, bones, implants; chronic & non healing present a huge challenge. They need intense management with multiple surgical intervention, antimicrobial /antibiotics, analgesics, and local care. In this clinical intervention, study a comprehensive solution based on regenerative medicine product autologous Platelet Rich Plasma (PRP) is clinically tested on 250 wounds. A protocol has been developed - STARS therapy (Sandeep’s technique for assisted regeneration of skin). The results reveal a complete healing with control of infection, filling up of defects and assisted regeneration of skin. For the first time, reversal and revival of grossly necrosed and devitalised tissues such as tendons, muscles and bones has been clinically achieved. With development of regenerative medicine, we are looking into an exciting option and possibilities. The PRP is one of the most simplest Regenerative medicine products having huge possibility of reproducibility amongst all health settings across the globe. A comprehensive effective mono-therapy solution based on the PRP only, will have a huge impact in improving wound care across the globe. This “STARS therapy” is a simple safe and effective, such solution. It is a feasible management intervention for every health worker and settings, requiring minimal resource and training. It paves the way for future wound management and has potential to be a complete Game Changer benefiting hugely scores of patients suffering from such conditions and its morbidity

Biography:

Francesca Uberti graduated from University of Eastern Piedmont, UPO Novara (Italy), Biotechnology in 2004. She had master Medical and Pharmaceutical biotechnology degree in the same university in 2006. She completed PhD program in 2010 and then started working at Dept. Translational Medicine Physiology Lab, UPO. She is professor of General Pathology and Biochemistry at Cattolica University (Italy) since 2015. She has win two-time a Italian grant “Master dei Talenti”in 2015 and 2016. She is a co-founder and CEO&CTO of noiVita srls, a spin-off and start-up of UPO University since 2015. She has twenty-three articles published in international scientific journals

Abstract:

The physiological effects of acetylcholine on keratinocytes depend on the presence of nicotinic and muscarinic receptors. The role of nonneuronal acetylcholine in keratinocytes could have important clinical implications for patients with various skin disorders. In order to evaluate the efficacy of highly diluted acetylcholine solutions obtained by sequential kinetic activation, we aimed to investigate the effects of these solutions on normal human keratinocytes. Two different concentrations (10 fg/mL and 1 pg/mL) and formulations (kinetically activated and nonkinetically activated) of acetylcholine were used to verify keratinocyte viability, proliferation, and migration and the intracellular pathways involved using MTT, crystal violet, wound healing, and Western blot compared to 147 ng/mL acetylcholine. The activated formulations (1 pg/mL and 10 fg/mL) revealed a significant capacity to increase migration, cell viability, and cell proliferation compared to 147 ng/mL acetylcholine, and these effects were more evident after a single administration. Sequential kinetic activation resulted in a statistically significant decrease in reactive oxygen species production accompanied by an increase in mitochondrial membrane potential and a decrease in oxygen consumption compared to 147 ng/mL acetylcholine. The M1 muscarinic receptor was involved in these effects. Finally, the involvement of ERK/MAPK and KI67 confirmed the effectiveness of the single treatment on cell proliferation. The intracellular pathways of calcium were investigated as well. Our results indicate for the first time that highly diluted and kinetically activated acetylcholine seems to play an active and safe role in an in vitro model of wound healing.

Biography:

Pande Abasi is currently undertaking a Master’s Degree at the age of 25 years from Mountains of the Moon University where he volunteers as Student Lecturer. He is the Project director of Teens Sports Development Agency, a premier Social work service organization. He has published more than 15 papers in different Newspapers and offered his work to the all continent of Africa.

Abstract:

Biological studies have always constituted a large pool of inspiration for the design of systems.

In the last decades, two biological systems have provided a remarkable source of inspiration for the development of new types of algorithms: neural networks and evolutionary algorithms.

In recent years, another biological inspired system has attracted the attention of researchers, the immune system and its powerful information processing capabilities. In particular, it performs many complex computations in a highly parallel and distributed fashion. The key features of the immune system are: pattern recognition, feature extraction, diversity, learning, memory, self- regulation, distributed detection, probabilistic detection, adaptability, specificity, etc. The mechanisms of the immune system are remarkably complex and poorly understood, even by immunologists. Several theories and mathematical models have been proposed to explain the immunological phenomena. There is also a growing number of computer models to simulate various components of the immune system and the overall behavior from the biological point.

Biography:

Krishna Debbarman has completed his MD at the age of 27 years from All India Institute of Medical Sciences, New Delhi. He is the faculty of Maulana Azad Medical College, Delhi University, Delhi, India. He has published more than 20 papers in reputed journals and has been reviewer of many reputed journals.

Abstract:

Aim: Various surgical procedures are available for management of acne scars and they can be combined with variable success. As micro needling targets superficial boxcar scars while subcision targets primarily rolling scars, it can be assumed that a combination of the two would give better results. Since no data available about the efficacy of micro needling alone vs microneedling with subcision in management of post acne scarring, this study was performed to compare the efficacy of both the procedures.

Material and Methods: A total of 40 patients with post acne scars were included in the study and were alternately recruited into two groups; Group-A (Microneedling) and Group-B (Combined Microneedling and Subcision) and the procedures were performed under aseptic precaution. All cases were followed on monthly intervals. The improvement was evaluated on VAS by both physician and patient accordingly. Appropriate statistics were used to analyze the results.

Results: Out of 40 patients age ranging from 18-44 years with a mean of 25.80 years, 37 patients completed the study. Majority (60%) were in the age of 20-30 years and the ratio (M:F) was equal. The mean number of rolling acne scars in Group-A decreased by 8.6% as compared to 27.9% in Group-B. There was a significant statistical difference. The mean number of icepick scars in Group-A decreased by 4.9% as compared to 18.25% in Group-B. The mean number of boxcar scars in Group-A decreased by 2.91% as compared to 18.32% in Group-B and a statistical significant difference was achieved between baseline and 6th visit (p-.048). The mean number of total acne scars decreased by 4.83%  and 19.65% respectively in group A & B over a 6 months periods. However, there was no significant statistical difference. No significant adverse effects were noted in any of the patients, except postinflammatory hyperpigmentation in one patient.

The findings indicate that both microneedling and combination of microneedling with subcision produce good results. But combination gives faster and superior results when compared to microneedling alone in treating rolling and boxcar scars.

Biography:

Alper TuÄŸral has graduated as a Physiotherapist from Hacettepe University, Faculty of Health Sciences, Physical Therapy and Rehabilitation. He started working at Abant Izzet Baysal University (AIBU) as a research assistant in 2015. In the same year, he was certified by FoldiSchule, Germany. Later he obtained his master’s degree from AIBU Institute of Health Sciences at 2016 with the subjects of lower limb lymphedema. He is a PhD student now and continues his researches at the same Institute. His working specialties are on breast cancer, breast and gynecologic cancer-related lymphedema, chronic venous insufficieny, wound care, exercise-based intervention in oncologic rehabilitation. Presently he has been working at AIBU in Bolu.

Abstract:

Normal skin structure shows a major affection in lower limb lymphedema along with it is a structure complex carries primary importance in protective barrier function and integration natural moisture balance. Integration natural moisture balance of skin should be placed primarily in the treatment plan against the corruption of skin integrity, injuries, infections and as the most importantly to recurrences. In lower limb lymphedema, skin is more fragile and vulnerable to injuries due to prolonged and increased interstitial pressure along with increased diffusion distance and ineffective nourishment. Adipose tissue and fibrotic changes in basal are reported as the underlying reason, therefore these changes could cause advanced skin changes. In lower limb lymphedema, erysipelas infection which might be experienced by patients due to corrupted immune function and inadequate barrier function of skin was reported as 29% in chronic edema. Above 25% of this population, hospitalization was reported as a requirement1. According to the National Health Service (NHS), 96 million £ health expense and 7.1 days hospitalization in average was reported for erysipelas infection2. Researchers reported that stabilization should be integrated with broad range antibiotics along with the integration and continuity to complex decongestive. It is also reported that this approach is beneficial for reducing infection attacks and damage of lymphatics because of infection3. In addition, hyperkeratosis, papillomatosis, lymph fistule, fungal infections, folliculitis, ulcerations, and skin folds which are frequently encountered in lower limb lymphedema are critical points should urge upon in the treatment of lymphedema, especially in skin care.

Biography:

Ylfete A Shatri – Muçaj is a dermatovenerology and clinical pharmacology specialist. For specialization of clinical pharmacology is one of the first generations for basic specialization in this field in Balkans. She finished post-graduation and specialization studies in the University of Novi Sad. Currently, she works in UCC in Prishtina from 1981; as a specialist of clinical pharmacology she has treated for 4 years patients with chemotherapy near Internal Clinic – Department of Hematology in UCC. She led for many years the laboratory for magisterial preparations in the Center Pharmacy and Dermatovenerology Clinic near CUU in Prishtina. She worked for some years in the Institute of Biochemistry near Diagnostic Center of CUU. Since 2001 she is working as a Clinical Dermatovenerologist and Pharmacologist in KDV and now leads Dermatological polyclinic and laboratory for magisterial preparations for several skin diseases and and aesthetic issues by which she treats patients in the polyclinic “GENTIANA GreLorGen”. She participated in many conferences and congresses in region and Europe with several scientific works in the field of clinical Dermatology, Oncology and Pharmacology and also as a lecturer of chemotherapy in the National Congress of Oncologists in Prishtina. She is a member of EADV and Kosovo Association of Dermatovenerologists and Oncologists.

Abstract:

Skin changes in the surface of the whole body are extremely problematic for both male and female patients, but the most problematic and complex region is the face (because of its exposure in different factors).

Therefore, from my personal experience, I saw it necessary and reasonable to research and compile a very complex prescription, such as personal magistral preparations: Loregen Sun Day and Previta Gen.

These creams, contain a combination of moisturizing, epithelizing, astringent, revitalizing, vitamins (Vit.C, A, D, E), additional adjuvants, flavoring and cream base for magisterial preparation.

As for active substance, both of the creams contain Antipirin (Phenazone; Antipyrin; Analgesine;  - C11H12N2O 188.23g/mol) 20% and 10%, which thought its mechanism of action as inhibitor of prostaglandins, acts as an anti-inflammatory and at the same time serves as an astringents without leaving the skin to become more rude and  hyperpigmentated.

In my research, there are treated 334 patients of different ages from both sexes :( of them 203 female or 60.77% and 131 male or 39.22%).

These numbers of patients, depending on the changes of the skin of the faces are divided in three groups:

1.      Group one (I-st) with hyperpigmentation changes from different etiology.

2.      Group two (II-nd) with erythematosus changes, LED, Dermatitis, seborrhoica, ciakatrices post acne.

3.      Group three (III-rd) with Actenic Keratosis, Senil Keratosis, Verruacae et Keratoais seborrohica

Conclusions: The use of an automated PRO platform for mental health screening enables a clinician to identify patients at risk that may have otherwise gone unidentified. Our automated platform screened more unique patients than a manual process in the same time frame. The implementation of the automated process is implemented in just under 70% of our total health system’s appointments. By implementing automated PROs, we hope to identify our total population of patients at risk for mental health conditions.

Biography:

Shahryar Eghtesadi  received Bachelor degree in Nutrition Science and Food Chemistry 1975, from Shahid Beheshti University of Medical Sciences, Tehran; MSPH degree in Nutrition, 1977, from Tehran University of Medical Sciences, Tehran and PhD from University of  California at Davis(UCD), USA, in Nutrition (1985). He served as Visiting Scientist in USDA Human Nutrition Research Center on Aging (HNRCA), Boston, USA (1994-1995); full professor of Tabriz, Iran and Tehran Universities of Medical Sciences and currently serves as Professor of Azad University, Science & Research Branch. He was the chairs of Departments of Nutrition and Biochemistry, Biochemistry & Clinical Nutrition, Public Health Nutrition and Nutrition in aforementioned Universities. Also Served as Associate Dean and Dean of School of Public Health & Nutrition and School of Public Health of Tabriz and Iran Universities of Medical Sciences respectively. He was selected as distinguished professor and Scientist. For long and extended period of time, experienced teaching various courses in nutrition in undergraduate, graduate and postgraduate and international Bureau programs and directed many projects and dissertation of MS and PhD programs and Published numerous peer reviewed articles in journals and also edited several books and finally served as Principal Investigator of World Bank Project for Capacity Building in Nutrition in Iran.

Abstract:

Sepsis is one of the major causes of death in intensive care units. Oxidative stress and hyper-inflammation has been shown to be major cause of mortality and morbidity in septic cases. Pomegranate is a fruit which is considered for its antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effect of POMx, a standard pomegranate extract, on mortality and peritoneal bacterial load in cecal ligation and perforation (CLP) model of sepsis in rats. Male wistar rats were divided into four groups: sham; CLP; prevention [consumed POMx (250mg of polyphenols/kg/day) for 4 weeks and subjected to CLP]; treatment [subjected to CLP and then received a single drink of POMx (250mg of polyphenols/kg)]. Sepsis was induced by CLP surgery. Ten day survival rate of all groups (subdivided into with and without antibiotics subgroups) were recorded. Peritoneal bacterial load of animal were also assessed.Data were analysed using log-rank and Kruskal-Wallis tests. There were no significant differences in survival rates of CLP, prevention and treatment groups, in subgroups without antibiotics. However, in subgroups with antibiotics, the prevention group had significantly lower survival rate than sham group (p<0.05). Conversely, the bacterial load of prevention and treatment group were significantly higher than sham group (p<0.01).In conclusion our study demonstrated that pomegranate extract could increase mortality rate via increasing peritoneal cavity bacterial load, in CLP model of sepsis. More studies to assess mechanisms of this effect are warranted.

Biography:

Yesim BAKAR graduated as a Physiotherapist from Hacettepe University, Faculty of Health Sciences, Physical Therapy and Rehabilitation. She started working at Hacettepe University (HU) as a research assistant in 1997. In 2001 she was certified as Lymphedema Therapist by Foldischule, Germany. She obtained her master’s degree at 2000 and Ph.D degree at 2005 from HU Institute with the subjects of breast cancer-related lymphedema. She has been working as Professor at Abant Izzet Baysal University School of Physical Therapy and Rehabilitation since 2006. Her working specialties are on breast and gynecologic cancer-related lymphedema, chronic venous insufficiency, wound care.

Abstract:

In Europe, venous leg ulcers are the most common cause of chronic wounds. Chronic venous insufficiency is the most important pathophysiological factor in patients with chronic leg ulcers. Not only compression therapy represents the basis of all other therapeutic strategies for venous leg ulcers but also it is the most important component of Complex Decongestive Physiotherapy (CDP). CDP has two phases. Phase 1 is called as decongestion phase and consists of manual lymphatic drainage, skin care, compression bandages, and exercise. In the decongestion phase, the primary goal is edema reduction, improvement of microcirculation and healing of the venous ulcer. In this phase, compression bandages have to be changed daily. Optimal compression brings a decrease in wound exudate as well as in lower limb edema reduction. Phase two of CDP is the maintenance phase and it consists of manual lymphatic drainage, skin care, compression stockings, and exercise. In this phase, edema reduction has been initiated, and venous leg ulcers have frequently transitioned into a progressive healing process. The goal is the prevention of aggravation of edema and recurrences.

There are currently wide varieties of compression materials available. While especially short- stretch bandages should be used in the decongestion phase, compression stockings are recommended for the subsequent maintenance phase. Compression therapy plays a crucial role in the treatment of patients with venous leg ulcers. Optimal compression therapy in combination with exercise is the basis for successful treatment.

In recent years, Complex Decongestive Physiotherapy become available. This paper presents about Complex Decongestive Physiotherapy and the basic principles of compression therapy in patients with venous leg ulcers.

Biography:

Yuzhi Jiang graduated from the Second Military Medical University as Medical Doctor, with the specialties including Plastic surgery, Microsurgery. Then he started working at Shanghai burns institute, Ruijin Hosptial, Shanghai Jiao Tong University School of Medicine where he has continued his research.

Abstract:

The skin lines outside of human being and is the largest organ in the human being. When it is injured, it will affect the quality of human life, such as decreased self-esteem and poor self-perception, which are not beneficial for the physical and psychiatric health of human being. Currently, the problems are still not resolved. Therefore, it is imperative to find the ideal ways to clarify the mechanism. Clinic works and currently researches implicated the integrity and continuity of dermal tissues plays an important role in the wound healing. Researchers have known the difference between normal tissues and wound healed tissue, however, these information are based on the two-dimensional structure and not complete. A novel technique, phase-contrast micro-tomography with synchrotron radiation (PCTSR), showed that the normal tissue was consistently characterized by oval-shaped units oriented in a specific way on the micrometer scale, which was not observed in scar tissues. In addition, the previous study also showed that the non-wound skin from diabetes was different from the health human being and indicative of the chronic foot ulcer. In order to further explore the changes of the structure of skin, we tried to apply for the small angle X-ray scattering (SAXS) to observe the skin after wound healed.The results showed that there is difference between the normal tissue and abnormal tissue, there is even difference between the normal collagen fiber and fibers in different degree of burns, the diabetic. The results will be beneficial for skin tissue engineering, and wound regeneration.